Neurogranin Antikörper (Internal Region)

Produktnummer ABIN2464335

Produktdetails anti-Neurogranin Antikörper

Target: Neurogranin (NRGN)

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Neurogranin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Neurogranin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Neurogranin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Neurogranin.

Anwendungsinformationen

Applikationshinweise: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 15 kDa band observed in human brain (cerebral cortex and hippocampus), and in mouse brain and rat brain lysates (calculated MW of 7.6 kDa according to NP_006167.1). There is also an additional 16 kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15,274 (3) :1294-300, PMID: 9880498). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining in the neuronal cell bodies Recommended concentration, 4-6 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Details zu Neurogranin

Target: Neurogranin (NRGN)

Andere Bezeichnung: Neurogranin (NRGN Produkte)

Gen-ID: 4900

NCBI Accession: NP_006167

UniProt: Q92686