Vimentin Antikörper (truncated)

Produktnummer ABIN2464120

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vimentin in ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Vimentin Antikörper (truncated) (ABIN2464120)

Target: Vimentin (VIM)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: ELISA

Klon: 9E7E7-5G3F10

Produktdetails anti-Vimentin Antikörper

Bindungsspezifität: truncated

Immunogen: Ni-NTA purified truncated recombinant Vimentin expressed in E. Coli strain BL21 (DE3).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Lagerung: -20 °C

Informationen zur Lagerung: Vimentin monoclonal antibody can be stored at -20 °C, stable for one year.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose