EGLN3 Antikörper

Produktnummer ABIN2463514

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EGLN3 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für EGLN3 Antikörper (ABIN2463514)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGLN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-EGLN3 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN3.

Anwendungsinformationen

Applikationshinweise: EGLN3 antibody can be used for detection of EGLN3 by ELISA at 1:1562500. EGLN3 antibody can be used for detection of EGLN3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EGLN3 antibody at -20 °C.

Details zu EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Andere Bezeichnung: EGLN3

Hintergrund: EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.

Molekulargewicht: 27 kDa

Gen-ID: 112399

NCBI Accession: NP_071356

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity