DPH1 Antikörper

Produktnummer ABIN2463486

Der Kaninchen Polyklonal anti-DPH1 Antikörper (ABIN2463486) detektiert spezifisch DPH1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für DPH1 Antikörper (ABIN2463486)

Target: DPH1 (DPH1 Homolog (DPH1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DPH1 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH1.

Anwendungsinformationen

Applikationshinweise: DPH1 antibody can be used for detection of DPH1 by ELISA at 1:312500. DPH1 antibody can be used for detection of DPH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DPH1 antibody at -20 °C.

Details zu DPH1

Target: DPH1 (DPH1 Homolog (DPH1))

Andere Bezeichnung: DPH1

Hintergrund: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Molekulargewicht: 49 kDa

Gen-ID: 1801

NCBI Accession: NP_001374

UniProt: Q9BZG8