Splicing Factor 1 Antikörper

Produktnummer ABIN2462368

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Splicing Factor 1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Hund.

Kurzübersicht für Splicing Factor 1 Antikörper (ABIN2462368)

Target: Splicing Factor 1 (SF1)

Reaktivität: Human, Maus, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Splicing Factor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Splicing Factor 1 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1.

Anwendungsinformationen

Applikationshinweise: SF1 antibody can be used for detection of SF1 by ELISA at 1:62500. SF1 antibody can be used for detection of SF1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SF1 antibody at -20 °C.

Details zu Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Andere Bezeichnung: SF1

Hintergrund: SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.

Molekulargewicht: 68 kDa, 69 kDa

Gen-ID: 7536

NCBI Accession: NP_004621

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location