CPSF4 antibody can be used for detection of CPSF4 by ELISA at 1:62500. CPSF4 antibody can be used for detection of CPSF4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Konzentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handhabung
As with any antibody avoid repeat freeze-thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CPSF4 antibody at -20 °C.
Target
CPSF4
(Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))
cpsf30 antikoerper, nar antikoerper, neb1 antikoerper, 30kDa antikoerper, C79664 antikoerper, CPSF30 antikoerper, NAR antikoerper, NEB1 antikoerper, cleavage and polyadenylation specific factor 4 antikoerper, cleavage and polyadenylation specific factor 4, 30kDa antikoerper, cleavage and polyadenylation specific factor 4 S homeolog antikoerper, CPSF4 antikoerper, Cpsf4 antikoerper, LOC733099 antikoerper, LOC100360200 antikoerper, cpsf4.S antikoerper, cpsf4 antikoerper
Hintergrund
Inhibition of the nuclear export of poly (A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.