CPSF4 Antikörper

Produktnummer ABIN2462224

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CPSF4 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hund.

Kurzübersicht für CPSF4 Antikörper (ABIN2462224)

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPSF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CPSF4 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPSF4.

Anwendungsinformationen

Applikationshinweise: CPSF4 antibody can be used for detection of CPSF4 by ELISA at 1:62500. CPSF4 antibody can be used for detection of CPSF4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CPSF4 antibody at -20 °C.

Details zu CPSF4

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Andere Bezeichnung: CPSF4

Hintergrund: Inhibition of the nuclear export of poly (A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

Molekulargewicht: 24 kDa, 28 kDa, 30 kDa

Gen-ID: 10898

UniProt: O95639