NFYB Antikörper

Produktnummer ABIN2461778

Der Kaninchen Polyklonal Anti-NFYB-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, NFYB in Proben von Human, Maus, Ratte und Hund zu detektieren.

Kurzübersicht für NFYB Antikörper (ABIN2461778)

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-NFYB Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYB.

Anwendungsinformationen

Applikationshinweise: NFYB antibody can be used for detection of NFYB by ELISA at 1:312500. NFYB antibody can be used for detection of NFYB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYB antibody at -20 °C.

Details zu NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Andere Bezeichnung: NFYB

Hintergrund: NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.

Molekulargewicht: 23 kDa

Gen-ID: 4801

NCBI Accession: NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha