Antibody is purified by protein A chromatography method.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLIon Channel4.
Applikationshinweise
CLIon Channel4 antibody can be used for detection of CLIon Channel4 by ELISA at 1:62500. CLIon Channel4 antibody can be used for detection of CLIon Channel4 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Konzentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handhabung
As with any antibody avoid repeat freeze-thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CLIon Channel4 antibody at -20 °C.
Target
Cl Ion Channel 4
Hintergrund
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH , and regulation of cell volume. Chloride intracellular channel 4 (CLIon Channel4) protein, encoded by the CLIon Channel4 gene, is a member of the p64 family, the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).