Shugoshin Antikörper

Produktnummer ABIN2452123

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Shugoshin in WB und IF. Er zeigt eine Reaktivität gegenüber Schizosaccharomyces pombe und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Shugoshin Antikörper (ABIN2452123)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reaktivität: Schizosaccharomyces pombe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Shugoshin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-Shugoshin Antikörper

Aufreinigung: Affinity purified

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 1,000 fold dilution
2) Immunofluoresece staining: 100 fold dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 1 mg/mL BSA, PBS ( pH 7.2), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen für anti-Shugoshin Antikörper (ABIN2452123)

Yamagishi, Sakuno, Shimura, Watanabe: "Heterochromatin links to centromeric protection by recruiting shugoshin." in: Nature, Vol. 455, Issue 7210, pp. 251-5, (2008) (PubMed).

Watanabe, Kitajima: "Shugoshin protects cohesin complexes at centromeres." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 360, Issue 1455, pp. 515-21, discussion 521, (2005) (PubMed).

Kitajima, Kawashima, Watanabe: "The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis." in: Nature, Vol. 427, Issue 6974, pp. 510-7, (2004) (PubMed).

Details zu Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Hintergrund: Shugoshin 1 (Sgo1), a meiosis-specific protein, protects centromeric Rec8 from degradation during first meiotic division (meiosis I) in fission yeast Schizosaccharomyces pombe. This protein appears at late prophase of meiosis I and is degraded at anaphase I. Heterochromatin protein Swi6 associates directly with Sgo1. A point mutation of Sgo1 (V242E), which abolishes the interaction with Swi6, impairs the centromeric localization and function of Sgo1. The forced centromeric localization of Sgo1 restores proper meiotic chromosome segregation in swi6 cells. Using this antibody in Western blotting, the band of 37 kD was observed in wild type S. pombe MP111 extract.

UniProt: Q9P7A0

Pathways: Maintenance of Protein Location