NUP62 Antikörper (Phenylalanine-Glycine Repeat)

Produktnummer ABIN2452063

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch NUP62 in WB, ELISA, IP, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Affe und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für NUP62 Antikörper (Phenylalanine-Glycine Repeat) (ABIN2452063)

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Reaktivität: Human, Affe

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser NUP62 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 2A

Produktdetails anti-NUP62 Antikörper

Bindungsspezifität: AA 1-179, Phenylalanine-Glycine Repeat

Spezifität: Specific to human (HeLa cells) and simian (Cos cells). The antibody did not react with mouse.

Aufreinigung: Purified from serum-free culture medium of the hybridoma by proprietary chromatography under mild conditions.

Sterilität: Sterile filtered

Immunogen: Recombinant human Nup62 (amino acids 1-300) (GST-Nup62-His)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: 1/500 ~1/2,000 dilution
2. Immunoprecipitation (assay dependent)
3. Immunofluorescece / Immunocytochemistry: 1/400)
4. ELISA (assay dependent)
5. When this antibody was micro-injected into the cytoplasm of the HeLa cells, it accumulates into the nuclear pores as examined by immunofluorescence staining. .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen für anti-NUP62 Antikörper (ABIN2452063)

Fukuhara, Sakaguchi, Katahira, Yoneda, Ogino, Tachibana: "Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies." in: Hybridoma (2005), Vol. 25, Issue 2, pp. 51-9, (2006) (PubMed).

Maeshima, Yahata, Sasaki, Nakatomi, Tachibana, Hashikawa, Imamoto, Imamoto: "Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins." in: Journal of cell science, Vol. 119, Issue Pt 21, pp. 4442-51, (2006) (PubMed).

Details zu NUP62

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Andere Bezeichnung: Nup62

Hintergrund: The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa, 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain .

UniProt: P37198

Pathways: EGFR Signaling Pathway, SARS-CoV-2 Protein Interaktom