Nas6/p28 Antikörper

Produktnummer ABIN2452055

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Nas6/p28 Antikörper wird verwendet zum Nachweis von Nas6/p28 in Proben von Saccharomyces cerevisiae. Er wurde validiert für IP und WB.

Kurzübersicht für Nas6/p28 Antikörper (ABIN2452055)

Target: Nas6/p28

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Kreuzreaktivität (Details): Not tested in other species.

Aufreinigung: Affinity purified

Immunogen: Recombinant yeast Nas6 expressed in E. coli

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 5000 fold dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, 1 mg/mL BSA, 0.09 % sodium azide, 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen

Saeki, Toh-E, Kudo, Kawamura, Tanaka: "Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle." in: Cell, Vol. 137, Issue 5, pp. 900-13, (2009) (PubMed).

Hori, Kato, Saeki, DeMartino, Slaughter, Takeuchi, Toh-e, Tanaka: "cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome." in: Gene, Vol. 216, Issue 1, pp. 113-22, (1998) (PubMed).

Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, (1998) (PubMed).

Antigendetails

Target: Nas6/p28

Hintergrund: Background: The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. NAS6 (p28) is a proteasome-interacting protein which acts as a chaperon to assemble the base sub-complex of the 19S RP. NAS6 is composed of 226 amino acid residues with calculated molecular masses of 24.4kD.