CBX1 Antikörper (AA 176-185)

Produktnummer ABIN2452034

Der Kaninchen Polyklonal Anti-CBX1-Antikörper wurde für WB, IF und ChIP-seq validiert. Er ist geeignet, CBX1 in Proben von Human und Hamster zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für CBX1 Antikörper (AA 176-185) (ABIN2452034)

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Reaktivität: Human, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-CBX1 Antikörper

Bindungsspezifität: AA 176-185

Kreuzreaktivität (Details): Expected to react with mouse, chicken, zebra fish, Xenopus, Drosophila orthologs due to the sequence identity of the immunogen.

Produktmerkmale: The product is prepared by immunizing rabbit with the synthetic peptide CNEDDDKKDDKN including the C-terminal sequence (176-185) of human HP1beta and purified by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi.

Aufreinigung: Affinity purified

Sterilität: Sterile filtered

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 1/2,000~1/10,000
2) Immunofluorescence staining
3) Chromatin immunoprecipitation (ChIP)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.44 mg/mL

Buffer: 0.12 M sodium phosphate buffer ( pH 7.4), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -70 C)

Referenzen für anti-CBX1 Antikörper (ABIN2452034)

Lomberk, Wallrath, Urrutia: "The Heterochromatin Protein 1 family." in: Genome biology, Vol. 7, Issue 7, pp. 228, (2007) (PubMed).

Wang, Koyama, Nishida, Haraguchi, Reith, Tsukamoto: "The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4028-40, (2006) (PubMed).

Kametaka, Takagi, Hayakawa, Haraguchi, Hiraoka, Yoneda: "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 12, pp. 1231-42, (2002) (PubMed).

Details zu CBX1

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Andere Bezeichnung: HP1 beta/cbx1

Hintergrund: Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1beta is encoded by the Chromobox homolog 1 (CBX1) gene located on chromosome 17q21.32. HP1beta has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions.

UniProt: P83916