HHV6 gQ1 (AA 3-422) Antikörper

Produktnummer ABIN2452012

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IC, FACS, IF, IP, ELISA und WB. Er zeigt eine Reaktivität gegenüber Human Herpesvirus 6 (HHV-6) und wurde in 7+ Publikationen erwähnt.

Kurzübersicht für HHV6 gQ1 (AA 3-422) Antikörper (ABIN2452012)

Target: HHV6 gQ1

Reaktivität: Human Herpesvirus 6 (HHV-6)

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunochromatography (IC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)

Klon: 119

Produktdetails

Bindungsspezifität: AA 3-422

Spezifität: Reacts with gQ1 of HHV6A and HHV6B. However, this antibody is not recommended for IP of HHV-6B due to conformation specificity.

Aufreinigung: Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions.

Reinheit: 90-95 % pure by SDS-PAGE.

Sterilität: Sterile filtered

Immunogen: His6-tagged recombinant gQ1 of HHV-6A encoding 3-422 amino acids expressed in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 1/500~1/1,000 dilution
2) Immunoprecipitation (assay dependent)
3) Imunofluorescence staining and Immunocytochemistry: 1/100~1/3,200 dilution
4) Flow Cytometry: 1/100
5) ELISA (assay dependent)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen

Tang, Hayashi, Maeki, Yamanishi, Mori: "Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding." in: Journal of virology, Vol. 85, Issue 21, pp. 11121-30, (2011) (PubMed).

Tang, Kawabata, Yoshida, Oyaizu, Maeki, Yamanishi, Mori: "Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth." in: Virology, Vol. 407, Issue 2, pp. 360-7, (2010) (PubMed).

Huang, Li, Sadaoka, Tang, Yamamoto, Yamanishi, Mori: "Human herpesvirus 6 envelope cholesterol is required for virus entry." in: The Journal of general virology, Vol. 87, Issue Pt 2, pp. 277-85, (2006) (PubMed).

Mori, Akkapaiboon, Yonemoto, Koike, Takemoto, Sadaoka, Sasamoto, Konishi, Uchiyama, Yamanishi: "Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46." in: Journal of virology, Vol. 78, Issue 9, pp. 4609-16, (2004) (PubMed).

Akkapaiboon, Mori, Sadaoka, Yonemoto, Yamanishi: "Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope." in: Journal of virology, Vol. 78, Issue 15, pp. 7969-83, (2004) (PubMed).

Mori, Akkapaiboon, Yang, Yamanishi: "The human herpesvirus 6 U100 gene product is the third component of the gH-gL glycoprotein complex on the viral envelope." in: Journal of virology, Vol. 77, Issue 4, pp. 2452-8, (2003) (PubMed).

Mori, Yang, Akkapaiboon, Okuno, Yamanishi: "Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46." in: Journal of virology, Vol. 77, Issue 8, pp. 4992-9, (2003) (PubMed).

Antigendetails

Target: HHV6 gQ1

Substanzklasse: Viral Protein

Hintergrund: Background: Human herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). These closely related viruses are two of the nine herpesviruses known to have humans as their primary host. HHV-6A and HHV-6B are double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus. HHV-6A and HHV-6B infects almost all of the human populations tested. The overall nucleotide sequence identity between HHV- 6A and HHV-6B is 90 % and they are now classified as distinct species. HHV-6A has been described as more neurovirulent, and as such is more frequently found in patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6B primary infection is the cause of the common childhood illness exanthem subitum (also known as roseola infantum or sixth disease). Additionally, HHV-6B reactivation is common in transplant recipients, which can cause several clinical manifestations such as encephalitis, bone marrow suppression and pneumonitis. gQ1 encoded by the U100 gene of HHV6 is glycoprotein, complexes with gH, gL and gQ2 to form HHV6A ligand to CD46 receptor and HHV6B ligand to CD134 receptor. It is expressed in two different forms: an 80- kDa form (gQ1-80K) and a 74- kDa form (gQ1-74K) - only gQ1-80K, but not gQ1-74K, forms the ligand complex with gQ2, gH, and gL. It associates with lipid rafts.