Tubulin Antikörper (cleaved, EEVGVD Motif)

Produktnummer ABIN2444034

Der Kaninchen Polyklonal anti-Tubulin Antikörper (ABIN2444034) detektiert spezifisch Tubulin in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Rind (Kuh).

Kurzübersicht für Tubulin Antikörper (cleaved, EEVGVD Motif) (ABIN2444034)

Target: Tubulin (TUB)

Reaktivität: Human, Maus, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Tubulin Antikörper

Bindungsspezifität: cleaved, EEVGVD Motif

Verwendungszweck: Alpha-Tubulin cleaved by caspase-6 Rabbit Polyclonal Antibody

Spezifität: The antibody recognizes the sequence (EEVGVD) in tubulin protein. It is specific for Caspase 6 cleaved α-Tubulin. The antibody was tested in IHC and western blots of samples with Alzheimer disease (AD) (Bovine brain sample, human samples, cells lines, mice hippocampus and whole brains). When tested in null mice with IHC and WB, no immunoreactivity was observed.

Immunogen: EEVGVD peptide

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IHC 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 50 μL: WB 1:1000, IHC 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu Tubulin

Target: Tubulin (TUB)

Andere Bezeichnung: Tubulin

Hintergrund:

α-Tubulin cleaved by casp 6

Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of α- and β-tubulin. α-Tubulin cleaved by caspase-6 can be found in neurons, neurofibrillary tangles, neutrophil threads, and neuritic plaques in Alzheimer disease. α-Tubulin has been found to associate with the axonal microtubule-associated protein Tau, indicating that the cleavage of the functional α-Tubulin C-terminal domain by caspase-6 could alter the state of microtubules in neurons.

UniProt: Q71U36