ITK Antikörper (AA 1-26)

Produktnummer ABIN2443927

Dieser Maus Monoklonal Antikörper detektiert spezifisch ITK in WB, IP und EAA. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für ITK Antikörper (AA 1-26) (ABIN2443927)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Activity Assay (EAA)

Klon: 2F12

Produktdetails anti-ITK Antikörper

Bindungsspezifität: AA 1-26

Verwendungszweck: Itk (IL2-inducible T-cell kinase) Mouse Monoclonal Antibody (2F12)

Spezifität: A highly specific and sensitive antibody against Itk. The antibody recognizes both native and denatured Itk (amino acids 1-26). No detectable cross reactivity with other Itk family members such as Btk or Tec. S

Aufreinigung: Protein G purified

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100μL: WB 2 μg/mL. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Andere Bezeichnung: Itk (IL2-inducible T-cell kinase)

Hintergrund: IL2-inducible T-cell kinase (Itk) is located in cell membrane of T-cell lines and natural killer cell lines and plays a role in T-cell proliferation and differentiation. Defects in Itk are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1).

UniProt: Q08881

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung