Clathrin, Light Chain (N-Term) Antikörper

Produktnummer ABIN2443922

Der Kaninchen Polyklonal anti-Clathrin, Light Chain Antikörper wird verwendet zum Nachweis von Clathrin, Light Chain in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IF.

Kurzübersicht für Clathrin, Light Chain (N-Term) Antikörper (ABIN2443922)

Target: Clathrin, Light Chain

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: N-Term

Verwendungszweck: Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody

Spezifität: The antibody recognizes the N-terminal portion of Clathrin Light Chain

Aufreinigung: Immunogen affinity purified

Immunogen: peptide in N term

Anwendungsinformationen

Applikationshinweise: IF 1:100,WB 1:1000-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 200 μL: IF 1:100 to 1:500, WB 1:1000-1:2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Antigendetails

Target: Clathrin, Light Chain

Andere Bezeichnung: Clathrin Light Chain

Hintergrund:

Clathrin light chain A, Lca

Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.

UniProt: P09496