EpCAM Antikörper

Produktnummer ABIN2443916

Der Maus Monoklonal anti-EpCAM Antikörper wird verwendet zum Nachweis von EpCAM in Proben von Human und Maus. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für EpCAM Antikörper (ABIN2443916)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: 4A7

Produktdetails anti-EpCAM Antikörper

Verwendungszweck: Epcam ICD Mouse Monoclonal Antibody (4A7)

Spezifität: The antibody recognizes the intracellular domain of human EpCAM.

Aufreinigung: Protein G purified

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Hintergrund:

Epithelial Cell Adhesion Molecule Intracellular Domain

Epithelial cell adhesion molecule (EpCAM) is a 40- kDa epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

UniProt: P16422