HMGCR Antikörper (full length)

Produktnummer ABIN2443869

Der Kaninchen Polyklonal anti-HMGCR Antikörper (ABIN2443869) detektiert spezifisch HMGCR in WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für HMGCR Antikörper (full length) (ABIN2443869)

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGCR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-HMGCR Antikörper

Bindungsspezifität: full length

Verwendungszweck: HMG Co A reductase Rabbit Polyclonal Antibody

Spezifität: The antibody recognizes the HMG CoA reductase

Immunogen: Full length protein

Anwendungsinformationen

Applikationshinweise: WB 1:500,IF 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:500, IF 1:50-1:100. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Andere Bezeichnung: HMG Co A reductase

Hintergrund:

3-hydroxy-3-methyl-glutaryl-CoA reductase, HMGCR

HMG-CoA reductase (also known as 3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGCR) is a transmembrane glycoprotein involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis, it is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum. HMG-CoA reductase is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. HMG-CoA is inhibited by statin, a class of cholesterol-lowering drugs which lower plasma cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor.

UniProt: P51639

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha