Nucleobindin (full length) Antikörper

Produktnummer ABIN2443849

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Nucleobindin in ELISA, ICC, IF, IP und WB. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für Nucleobindin (full length) Antikörper (ABIN2443849)

Target: Nucleobindin

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Bindungsspezifität: full length

Verwendungszweck: Nucleobindin Rabbit Polyclonal Antibody

Spezifität: The antibody recognizes nucleobindin 1 (NucB1)

Aufreinigung: Immunogen affinity purified

Immunogen: Full length recombinant human nucleobindin protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μg: WB: 1:1000, IF: 1:150, EM-ICC: 1:50. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Antigendetails

Target: Nucleobindin

Hintergrund:

NUCB1, NUC, CALNUC

Nucleobindin 1 is a 461 amino acids ubiquitous protein with an N-terminal of 25 amino acids signal peptide responsible for its initial localization to the endoplasmic reticulum (ER). It has also been detected in the cytoplasm and in various subcellular structures such as the nucleus, mitochondria and the Golgi apparatus. Nucleobindin plays a role in a number of cellullar processes such as intracellular signaling, inflammation, autoimmunity, osteogenesis and cancer.

UniProt: Q02818