Aconitase 1 Antikörper (full length)

Produktnummer ABIN2443828

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Aconitase 1 in WB. Es zeigt Reaktivität gegenüber Proben von Human und Maus.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Aconitase 1 Antikörper (full length) (ABIN2443828)

Target: Aconitase 1 (ACO1)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aconitase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Aconitase 1 Antikörper

Bindungsspezifität: full length

Verwendungszweck: Iron Regulatory Protein 1 (IRP1) Rabbit Polyclonal Antibody

Spezifität: The antibody recognizes the gull length IRP1 protein

Immunogen: Full length protein

Anwendungsinformationen

Applikationshinweise: WB 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 50 μL: WB 1:2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Referenzen für anti-Aconitase 1 Antikörper (ABIN2443828)

Deng, Manz, Torti, Torti: "Iron-responsive element-binding protein 2 plays an essential role in regulating prostate cancer cell growth." in: Oncotarget, Vol. 8, Issue 47, pp. 82231-82243, (2017) (PubMed).

Details zu Aconitase 1

Target: Aconitase 1 (ACO1)

Andere Bezeichnung: Iron Regulatory Protein 1 (IRP1)

Hintergrund:

Iron-responsive element-binding proteins, IRE-BP, IRBP, IRP, IFR

The iron regulatory proteins IRP1 and IRP2 are involved in posttranscriptional regulation of cellular iron metabolism by binding to mRNA iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. IRP-1 is a bifunctional soluble protein that functions as an IRE-binding protein or as the cytoplasmic isoform of aconitase.

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis