HGS Antikörper (full length)

Produktnummer ABIN2443821

Der Maus Monoklonal anti-HGS Antikörper (Klon 19-5) (ABIN2443821) detektiert spezifisch HGS in WB, IHC, ICC und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Affe.

Kurzübersicht für HGS Antikörper (full length) (ABIN2443821)

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HGS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 19-5

Produktdetails anti-HGS Antikörper

Bindungsspezifität: full length

Verwendungszweck: Hrs (HGS) Mouse Monoclonal Antibody (19-5)

Spezifität: The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein

Aufreinigung: Protein G purified

Immunogen: Full length hrs-2 fusion protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 50 μL: IHC: 1:300, ICC: 1:200, WB: 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu HGS

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Andere Bezeichnung: Hrs (HGS)

Hintergrund:

Hepatocyte growth factor-regulated tyrosine kinase substrate, HGS, Protein pp110

Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS, also known as HGS) is a mammalian protein predominantly localized on early endosomes. Hrs interacts with many proteins involved in membrane trafficking, such as eps15, SNX-1 and SNAP-25. Hrs is implicated in early endosome fusion and may play a role in cargo sorting/trafficking to early and late endosome by recruiting clathrin. Hrs is also involved in intracellular signal transduction mediated by cytokines and growth factors.

UniProt: O14964

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation