anti-HRP Antikörper

Produktnummer ABIN2443813

Dieser Maus Monoklonal Antikörper detektiert spezifisch HRP in IHC. Es zeigt Reaktivität gegenüber Proben von Meerrettich.

Kurzübersicht für anti-HRP Antikörper (ABIN2443813)

Target: HRP (Horseradish Peroxidase (HRP))

Reaktivität: Meerrettich

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (IHC)

Klon: MAP A6-2

Produktdetails anti-HRP Antikörper

Verwendungszweck: Horseradish Peroxidase (HRP) Monoclonal Antibody (MAP A6-2)

Spezifität: A sensitive antibody against horseradish peroxidase (HRP). This antibody was raised against the full horseradish peroxidase (HRP) protein

Aufreinigung: Protein G purified

Immunogen: HRP protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: IHC 1:30

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: IHC 1:30 - 1:60. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu HRP

Hintergrund: Horseradish peroxidase (HRP) is a 40 kDa glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and immunohistochemistry. A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.