Nerve Growth Factor Antikörper

Produktnummer ABIN2443811

Der Ratte Monoklonal anti-Nerve Growth Factor Antikörper wird verwendet zum Nachweis von Nerve Growth Factor in Proben von Maus. Er wurde validiert für WB, ELISA und IF.

Kurzübersicht für Nerve Growth Factor Antikörper (ABIN2443811)

Target: Nerve Growth Factor (NGF)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Klon: NGF30

Produktdetails anti-Nerve Growth Factor Antikörper

Verwendungszweck: Nerve growth Factor (NGF) Rat Monoclonal Antibody (NGF30)

Spezifität: This antibody recognizes mouse NGF monomer protein (MW 13 kDa) by WB. It does not cross react with EGF, BSA, APO or insulin.

Aufreinigung: Protein G purified

Immunogen: polymerized NGF

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:200. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Hintergrund:

Beta-nerve growth factor, Beta-NGF, NGF

Nerve growth factor (NGF) is a small secreted protein which induces the differentiation and survival of particular target neurons. It is perhaps the prototypical growth factor, in that it is one of the first to be described. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There is some data that shows that NGF can be transported from the axon tip to soma. One of the most important molecules in the nervous system, nerve growth factor and its other neurotrophin family members control the development of the nervous system in the embryo and the maintenance of nervous tissue and neural transmission in the adult.

UniProt: P01139

Pathways: Regulation of Cell Size