tau Antikörper (pTyr18)

Produktnummer ABIN2443721

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-tau Antikörper wird verwendet zum Nachweis von tau in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für tau Antikörper (pTyr18) (ABIN2443721)

Target: tau (tau Protein)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser tau Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 9G3

Produktdetails anti-tau Antikörper

Bindungsspezifität: AA 12-24, pTyr18

Verwendungszweck: Phospho-Tau (tyr 18) Mouse Monoclonal Antibody (9G3)

Spezifität: The antibody recognizes the human Phospho-Tau protein at the tyrosine 18 phosphorylation site with no detectable cross-reactivity.

Aufreinigung: Protein G purified

Immunogen: Peptide

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB 1:1000-1:5000,IHC 1:1000-1:5000,IF 1:2000-1:5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-1:5000, IHC 1:1000-1:5000, IF 1:2000-1:5000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Referenzen für anti-tau Antikörper (ABIN2443721)

Tang, Fesharaki-Zadeh, Takahashi, Nies, Smith, Luo, Chyung, Chiasseu, Strittmatter: "Fyn kinase inhibition reduces protein aggregation, increases synapse density and improves memory in transgenic and traumatic Tauopathy." in: Acta neuropathologica communications, Vol. 8, Issue 1, pp. 96, (2020) (PubMed).

Details zu tau

Target: tau (tau Protein)

Andere Bezeichnung: tau

Hintergrund:

Phosphorylated Tau

Tauopathies are a group of diverse dementias and movement disorders which have a common pathological feature in that they all display the presence of intracellular accumulation of abnormal filaments of Tau protein. While the abnormal phosphorylation of Tau on serine and threonine has been well established in the disease process, its phosphorylation on tyrosine 18 has only recently been described as present in Alzheimer's disease (AD) brain.

UniProt: P10636