His Tag Antikörper (C-Term, N-Term)

Produktnummer ABIN2443717

Dieser Maus Monoklonal Antikörper detektiert spezifisch His Tag in WB, ELISA, IP, IHC und DB. Es wurde in 1 Publikation zitiert.

Kurzübersicht für His Tag Antikörper (C-Term, N-Term) (ABIN2443717)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)

Klon: HIS-H8

Produktdetails anti-His Tag Antikörper

Bindungsspezifität: C-Term, N-Term

Verwendungszweck: His Tag Mouse Monoclonal Antibody (HIS.H8)

Sequenz: HHHHHH

Spezifität: The antibody recognizes His-tag encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags)

Aufreinigung: Protein G purified

Immunogen: The antibody was raised against HHHHHH (6x His) synthetic peptide conjugated to KLH.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IHC 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Referenzen für anti-His Tag Antikörper (ABIN2443717)

Bouvette, Korkut, Fouillen, Amellah, Nanci, Durocher, Omichinski, Legault: "High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension." in: BMC biotechnology, Vol. 18, Issue 1, pp. 76, (2019) (PubMed).

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: Epitope tags have applications in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size, the protein's biochemical properties seem not to be affected by the tag protein. Polyhistidine-tag is also known as histidine-tag, 6xHis-tag and by the trademarked name His-tag.