Myogenin Antikörper (AA 30-224)

Produktnummer ABIN241574

Der Maus Monoklonal anti-Myogenin Antikörper wird verwendet zum Nachweis von Myogenin in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IHC (p), ICC und IHC (fro).

Kurzübersicht für Myogenin Antikörper (AA 30-224) (ABIN241574)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myogenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 5FD

Produktdetails anti-Myogenin Antikörper

Bindungsspezifität: AA 30-224

Spezifität: The 5FD clone has been highly published, users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kD band should be observed. The can be used for gel shift assays.

Aufreinigung: Protein G purified

Immunogen: A recombinant GST fusion protein corresponding to amino acids 30-224 of rat myogenin.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (5 μg/mL), IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Short term: 4°C
Long term: -70°C.

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: MYOG / Myogenin

Hintergrund: Name/Gene ID: MYOG

Synonyms: MYOG, BHLHc3, Myogenin, MYF4, Myogenic factor 4, Myf-4, Myogenin (myogenic factor 4)

Gen-ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development