NCEH1 Antikörper (C-Term)

Produktnummer ABIN238671

Dieses Anti-NCEH1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von NCEH1 in ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für NCEH1 Antikörper (C-Term) (ABIN238671)

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NCEH1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-NCEH1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: AADACL1

Sequenz: RTRNSYIKWL DQN

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments gave an approx 26-28 kDa band in A431 cell lysates and in Human Hippocampus lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:1000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended con

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NCEH1

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Andere Bezeichnung: AADACL1

Hintergrund: AADACL1, arylacetamide deacetylase-like 1

Gen-ID: 57552, 320024

NCBI Accession: NP_065843, NP_001139748, NP_001139749