EPB41L5 Antikörper (Internal Region)

Produktnummer ABIN238631

Der Ziege Polyklonal anti-EPB41L5 Antikörper wird verwendet zum Nachweis von EPB41L5 in Proben von Human. Er wurde validiert für WB, ELISA, FACS und IF.

Kurzübersicht für EPB41L5 Antikörper (Internal Region) (ABIN238631)

Target: EPB41L5 (Erythrocyte Membrane Protein Band 4.1 Like 5 (EPB41L5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EPB41L5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-EPB41L5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: EBPL41L5

Sequenz: ENLPQSPGTD QHD

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ENLPQSPGTDQHD, from the internal region of the protein sequence according to NP_065960.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 80 kDa band observed in lysates of cell line A431 and approx. 75 kDa in lysates of cell line MCF7 (calculated MW of 81.9 kDa according to NP_065960.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperatur

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in U2OS and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu EPB41L5

Target: EPB41L5 (Erythrocyte Membrane Protein Band 4.1 Like 5 (EPB41L5))

Andere Bezeichnung: EPB41L5

Hintergrund: EPB41L5, erythrocyte membrane protein band 4.1 like 5, BE37, FLJ12957, KIAA1548

Molekulargewicht: 81.9kDa

Gen-ID: 57669

NCBI Accession: NP_065960