EPB41L2 Antikörper (Internal Region)
Quick Overview for EPB41L2 Antikörper (Internal Region) (ABIN238594)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
-
-
Bindungsspezifität
- Internal Region
-
Verwendungszweck
- EPB41L2 / 4.1G (aa 593 to 604)
-
Sequenz
- RREVRSPTKA PH
-
Spezifität
- This antibody is reported to recognise all isoforms NP_001422.1 (iso a), NP_001129026.1 (iso b), NP_001186317.1 (iso c), NP_001239589.1 (iso d), NP_001337228.1 (iso e), NP_001337230.1 (iso f), NP_001337232.1 (iso g),NP_001337233.1 (iso h), NP_001337236.1
-
Kreuzreaktivität
- Human
-
Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
Immunogen
- Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1.
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
-
Immunohistochemistry: Paraffin embedded Human Uterus and Colon. Recommended concentration: 2.5 μg/mL.
Western Blot: Preliminary testing showed bands at approx 110+40 kDa in Human Cerebellum lysatesafter 0.1 μg/mL antibody staining (calculated MW of 113 kDa according to NP_001422.1 These bands were successfully blocked by incubation with the immunizing pept
Peptide ELISA: antibody detection limit dilution 1:32000.
-
Kommentare
-
Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Re
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- 0.5 mg/mL
-
Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- Minimize freezing and thawing.
-
Lagerung
- -20 °C
-
Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- EPB41L2 (erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2))
-
Andere Bezeichnung
- EPB41L2
-
Hintergrund
- EPB41L2, 4.1-G, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2
-
Gen-ID
- 2037
-
NCBI Accession
- NP_001422
Target
-