ACRV1 Antikörper

Produktnummer ABIN238420

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ACRV1 in WB, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für ACRV1 Antikörper (ABIN238420)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACRV1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: Hs-14

Produktdetails anti-ACRV1 Antikörper

Spezifität: The antibody Hs-14 reacts with a 220 kDa testis-specific intra-acrosomal protein associated with the membranes of the acrosomal vesicle.

Produktmerkmale: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Aufreinigung: Precipitation methods and size-exclusion chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Western Blotting. Immunocytochemistry: 10 μg/mL, Staining technique: Membrane permeabilization(acetone) is essential. Application note: The antibody Hs-14 is designed for quantitativeimmunofluorescence analysis of pathological sperms (excellent tool for laboratories ofassisted reproduction when optimal method of fertilization is sought).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Andere Bezeichnung: ACRV1

Hintergrund: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2

Gen-ID: 56

UniProt: P26436