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Orf1 Antikörper

Reaktivität: Neisseria meningitidis WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN238346
  • Target
    Orf1
    Reaktivität
    • 5
    • 4
    Neisseria meningitidis
    Wirt
    • 9
    Kaninchen
    Klonalität
    • 9
    Polyklonal
    Konjugat
    • 3
    • 2
    • 2
    • 2
    Unkonjugiert
    Applikation
    Western Blotting (WB)
    Aufreinigung
    Protein-A affinity chromatography.
    Immunogen
    Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
  • Applikationshinweise
    Suitable for Western Blotting under Reducing conditions (0.2-1 μg/mL).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4 with 15 mM sodium azide as preservative.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Orf1
    Substanzklasse
    Viral Protein
    Hintergrund
    Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
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