Shows only the Scl-70 precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong Scl-70 precipitate band. Product shows no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen in an ELISA system designed to detect all IgG subclasses.
Produktmerkmale
Human anti Human Scl-70 Ag Human antibody to Human Scl-70 antigen
Aufreinigung
Single donor human plasma is delipidized and defibrinated. An immunoglobulin fraction is then precipitated and collected. Human plasma
Reaktivität: Human
ELISA, WB
Wirt: Maus
Monoclonal
4j81
unconjugated
Applikationshinweise
Suitable for use in Immunodiffusion and ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with 2 mL deionized water.
Buffer
Lyophilized from 0.01 M PBS, 0.11 M Sodium chloride, pH 7.2, 0.02 % Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.