Shows only the RNP precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong RNP precipitate band in double diffusion. No reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Scl-70, Sm, and Jo-1 antigens in an ELISA system designed to detect all IgG subclasses.
Produktmerkmale
Human anti Human RNP antigen Human antibody to Human RNP antigen
Aufreinigung
Single donor human plasma is delipidized and defibrinated. An immunoglobulin fraction is precipitated and collected. Human plasma.
Reaktivität: Human
ID, EIA, WB
Wirt: Human
Polyclonal
unconjugated
Applikationshinweise
Suitable for use in double immunodiffusion and ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with 2 mL of deionized water
Buffer
Lyophilized from 0.01 M Phosphate, 0.11 M Sodium chloride, pH 7.2, 0.02 % Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.