CD15 (Granulocytes) Antikörper (Biotin)

Produktnummer ABIN234527

Der Maus Monoklonal Anti--Antikörper wurde für FACS, IHC (f) und IFA validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für CD15 (Granulocytes) Antikörper (Biotin) (ABIN234527)

Target: CD15 (Granulocytes)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Indirect Immunofluorescence Assay (IFA)

Klon: TG1

Produktdetails

Spezifität: Recognizes a (Mr 156 kDa) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes.(1,2,3) The CD15 antigen is expressed on approximately 95% of human peripheral blood eosinophils and neutrophils and is present on approximately 20% of mature circulating monocytes. Anti-CD15 has virtually no reactivity with T or B cells.(1,2,3)

Produktmerkmale: MAb to CD15 Granulocytes, Monoclonal antibody to CD15 (Granulocytes) Biotin conjugated

Aufreinigung: Ion Exchange Chromatography and gel filtration. Tissue culture

Immunogen: Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1M glucose

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Suitable for use in flow cytometry, IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.01 M PBS, pH7.2 containing 1 % BSA, 0.1 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Antigendetails

Target: CD15 (Granulocytes)