PIN1 Antikörper (Internal Region)

Produktnummer ABIN233831

Hersteller Produkt- Nr.: 600-401-a20

Produktdetails anti-PIN1 Antikörper

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 600-401-a20

Hersteller: Rockland

Verwendungszweck: PIN1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Pin1.

Produktmerkmale: Synonyms: rabbit anti-PIN! antibody, PIN-1, PIN 1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody, Peptidyl-prolyl cis-trans isomerase Pin1, Prolyl isomerase antibody, Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody, Protein NIMA interacting 1 antibody, Rotamase Pin1 antibody, PPIase Pin1 antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Pin1.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 18 kDa in size corresponding to Pin1 by western blotting in the appropriate cell lysate or extract.  Lysates from 3T3, Jurkat, 293 or HeLa cells, as well as HeLa nuclear extract, are recommended for use as positive controls.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,500 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-PIN1 Antikörper (ABIN233831)

Lucchetti, Caligiuri, Toffoli, Giordano, Rizzolio: "The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55355, (2013) (PubMed).

La Montagna, Caligiuri, Maranta, Lucchetti, Esposito, Paggi, Toffoli, Rizzolio, Giordano: "Androgen receptor serine 81 mediates Pin1 interaction and activity." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 18, pp. 3415-20, (2013) (PubMed).

Rizzolio, Lucchetti, Caligiuri, Marchesi, Caputo, Klein-Szanto, Bagella, Castronovo, Giordano: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).

Details zu PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Andere Bezeichnung: PIN1 (PIN1 Produkte)

Hintergrund: Background: Pin1 (peptidylprolyl cis/trans isomerase NIMA-interacting 1 protein) is an essential peptidylprolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity.  Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond and also catalyzes pSer/Thr-Pro cis/trans isomerizations.  Pin1 shows a nuclear localization.

Gen-ID: 5300, 5453898

UniProt: Q13526