SEPHS2 Antikörper

Produktnummer ABIN233801

Hersteller Produkt- Nr.: 200-401-999

Der Kaninchen Polyklonal Anti-SEPHS2-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, SEPHS2 in Proben von Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für SEPHS2 Antikörper (ABIN233801)

Target: SEPHS2 (Selenophosphate Synthetase 2 (SEPHS2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SEPHS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-SEPHS2 Antikörper

Hersteller Produkt- Nr.: 200-401-999

Hersteller: Rockland

Verwendungszweck: Selenophosphate Synthetase 2 Antibody

Kreuzreaktivität (Details): This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1.

Produktmerkmale: Synonyms: rabbit anti-Selenophosphate Synthetase 2 Antibody, rabbit anti-SPS 2 antibody, Selenide water dikinase 2 antibody, Selenium donor protein 2 antibody, SEPHS2 antibody

Aufreinigung: This product was purified by Protein A chromatography from monospecific antiserum.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This Protein A purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SEPHS2 Antikörper (ABIN233801)

Pitts, Kremer, Hashimoto, Torres, Byrns, Williams, Berry: "Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 46, pp. 15326-38, (2015) (PubMed).

Details zu SEPHS2

Target: SEPHS2 (Selenophosphate Synthetase 2 (SEPHS2))

Andere Bezeichnung: Sephs2

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase (SelD) catalyzes the conversion of selenium to selenophosphate which is required by a number of bacterial, archaeal and eukaryotic organisms for synthesis of selenocysteine-tRNA, the precursor of selenocysteine in selenoenzymes.  A second selenophosphate synthetase (SPS2) was identified in mammals. SPS2 is itself a selenoprotein in mammals.

Gen-ID: 20768, 14717785

UniProt: P97364