Nuclear Factor kappa B p50 (NFkB p50) (N-Term) Antikörper

Produktnummer ABIN1043699

Hersteller Produkt- Nr.: 100-4164s

Produktdetails

Target: Nuclear Factor kappa B p50 (NFkB p50)

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Gel Shift (GS)

Hersteller Produkt- Nr.: 100-4164s

Hersteller: Rockland

Verwendungszweck: NFkB p50 Antibody

Kreuzreaktivität (Details): Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins.

Produktmerkmale: Synonyms: rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise:

Gel Shift Dilution: 0.5 to 1.0 μL

Application Note: This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  No reaction was observed against the analogous mouse protein.  This product was also tested in a gel supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 μL per assay.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Hariri, Arguello, Volpon, Culjkovic-Kraljacic, Nielsen, Hiscott, Mann, Borden: "The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia." in: Leukemia, Vol. 27, Issue 10, pp. 2047-55, (2013) (PubMed).

Ghosh, Yang, Rothstein, Robinson: "Nuclear factor-κB contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 25, pp. 9159-69, (2011) (PubMed).

Antigendetails

Target: Nuclear Factor kappa B p50 (NFkB p50)

Andere Bezeichnung: NFKB p50

Hintergrund: Background: Anti NFkB p50 Antibody recognizes NFKB p50 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.  It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Gen-ID: 4790

UniProt: P19838