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MHV NSP9 Antikörper

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in WB und FM. Geeignet für Mouse Hepatitis Virus (MHV).
Rockland
Produktnummer ABIN233751
Hersteller Produkt- Nr.: 200-301-a56

Kurzübersicht für MHV NSP9 Antikörper (ABIN233751)

Target

MHV NSP9

Reaktivität

Mouse Hepatitis Virus (MHV)

Wirt

  • 1
Maus

Klonalität

  • 1
Monoklonal

Konjugat

  • 1
Unkonjugiert

Applikation

Western Blotting (WB), Fluorescence Microscopy (FM)

Klon

2C6-H1
  • Hersteller Produkt- Nr.

    200-301-a56

    Hersteller

    Rockland

    Produktmerkmale

    Concentration Definition: by UV absorbance at 280 nm

    Immunogen

    This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
    Immunogentype:Native

    Isotyp

    IgG
  • Applikationshinweise

    This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA.  After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    2.1 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C
  • Target

    MHV NSP9

    Substanzklasse

    Viral Protein

    Hintergrund

    The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
    Synonyms: MHV-A59 nsp9 protein antibody

    Gen-ID

    1489749, 25121567

    UniProt

    P0C6V0
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