MHV NSP9 Antikörper
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- Target
- MHV NSP9
- Reaktivität
- Mouse Hepatitis Virus (MHV)
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
- Applikation
- Western Blotting (WB), Fluorescence Microscopy (FM)
- Hersteller Produkt- Nr.
- 200-301-a56
- Hersteller
- Rockland
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Immunogen
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This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native - Klon
- 2C6-H1
- Isotyp
- IgG
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- Applikationshinweise
- This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 2.1 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
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- Target
- MHV NSP9
- Substanzklasse
- Viral Protein
- Hintergrund
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The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody - Gen-ID
- 1489749, 25121567
- UniProt
- P0C6V0
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