DBH Antikörper (AA 40-57)

Produktnummer ABIN232869

Dieses Anti-DBH-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DBH in WB und IHC. Geeignet für Human.

Kurzübersicht für DBH Antikörper (AA 40-57) (ABIN232869)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DBH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-DBH Antikörper

Bindungsspezifität: AA 40-57

Spezifität: Recognizes Dopamine Beta Hydroxylase (DbH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band (s) of M. W. 70-75000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species cross-reactivity: By immunoblot: human, monkey (slight), sheep, canine (slight), rat and bovine. Also shown to react with equine ganglia cells.

Homologie: Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla (94%) Marmoset (89%) Mouse, Rat, Elephant, Porcine, Bovine (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide, C-SAPRESPLPYHIPLDPEG-amide, cloned from human (DbH) cDNA. Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla (94%), Marmoset (89%), Mouse, Rat, Elephant, Porcine, Bovine (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC (1:250 - 1:1000), WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:250-1:1000. Immunohistochemistry: 1:250-1:1000. Staining was evaluated in the locus ceruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed weak staining on both human and monkey.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Dulbecco's PBS (divalent-deficient), 10 % glycerol, 10 mM HEPES,  pH 7.2, 20 mM Tris-HCl,  pH 7.6, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Andere Bezeichnung: DBH/Dopamine Beta Hydroxylase

Hintergrund: Name/Gene ID: DBH
Family: Monooxygenase

Synonyms: DBH, Dopamine beta-monooxygenase, DBM, Dopamine beta-hydroxylase

Gen-ID: 1621

UniProt: P09172

Pathways: Carbohydrate Homeostasis