KAT2B Antikörper (AA 817-832)

Produktnummer ABIN229084

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch KAT2B in WB und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Hund, Rind (Kuh), Pferd, Affe und Fledermaus.

Kurzübersicht für KAT2B Antikörper (AA 817-832) (ABIN229084)

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Reaktivität: Human, Maus, Hund, Rind (Kuh), Pferd, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KAT2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-KAT2B Antikörper

Bindungsspezifität: AA 817-832

Spezifität: Recognizes human and canine PCAF. Species cross-reactivity: Predicted to react with mouse and chicken due to sequence homology.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%) Rat, Opossum, Platypus, Xenopus (94%) Chicken, Lizard, Zebrafish (88%) Stickleback, Pufferfish (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-terminal) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of human PCAF. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%), Rat, Opossum, Platypus, Xenopus (94%), Chicken, Lizard, Zebrafish (88%), Stickleback, Pufferfish (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB (1:500)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500, Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band (s) may appear. Staining of PCAF band (s) by immunoblotting is inhibited by the PCAF immunizing peptide. Immunoprecipitation: 5 μg immunoprecipitates PCAF from RIPA lysate with ~4x10^7 human acute T cell leukemia Jurkat cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 15 mM sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Andere Bezeichnung: KAT2B / PCAF

Hintergrund: Name/Gene ID: KAT2B

Synonyms: KAT2B, CREBBP-associated factor, GCN5, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, Lysine acetyltransferase 2B, Histone acetylase PCAF, PCAF, p300/CBP-associated factor, CAF, GCN5L

Gen-ID: 8850

UniProt: Q92831

Pathways: p53 Signalweg, Regulation of Carbohydrate Metabolic Process