KAT2B Antikörper (AA 817-832)

Produktnummer ABIN229084

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KAT2B in WB und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Hund, Rind (Kuh), Pferd, Affe und Fledermaus.

Kurzübersicht für KAT2B Antikörper (AA 817-832) (ABIN229084)

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Reaktivität: Human, Maus, Hund, Rind (Kuh), Pferd, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KAT2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-KAT2B Antikörper

Bindungsspezifität: AA 817-832

Spezifität: Recognizes human and canine PCAF. Species cross-reactivity: Predicted to react with mouse and chicken due to sequence homology.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%) Rat, Opossum, Platypus, Xenopus (94%) Chicken, Lizard, Zebrafish (88%) Stickleback, Pufferfish (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-terminal) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of human PCAF. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%), Rat, Opossum, Platypus, Xenopus (94%), Chicken, Lizard, Zebrafish (88%), Stickleback, Pufferfish (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB (1:500)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500, Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band (s) may appear. Staining of PCAF band (s) by immunoblotting is inhibited by the PCAF immunizing peptide. Immunoprecipitation: 5 μg immunoprecipitates PCAF from RIPA lysate with ~4x10^7 human acute T cell leukemia Jurkat cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 15 mM sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Andere Bezeichnung: KAT2B / PCAF

Hintergrund: Name/Gene ID: KAT2B

Synonyms: KAT2B, CREBBP-associated factor, GCN5, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, Lysine acetyltransferase 2B, Histone acetylase PCAF, PCAF, p300/CBP-associated factor, CAF, GCN5L

Gen-ID: 8850

UniProt: Q92831

Pathways: p53 Signalweg, Regulation of Carbohydrate Metabolic Process