MLANA Antikörper

Produktnummer ABIN228816

Dieses Anti-MLANA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MLANA in WB, FACS, IHC, IHC (p) und IP. Geeignet für Human und Pferd.

Kurzübersicht für MLANA Antikörper (ABIN228816)

Target: MLANA (Melan A (MLANA))

Reaktivität: Human, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLANA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-MLANA Antikörper

Spezifität: Recognizes MART-1 at 18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Species cross-reactivity: Human and equine. Does not cross-react with mouse or rat.

Aufreinigung: Protein A purified

Immunogen: Recombinant hMART-1 protein. MW: 18kD. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. (Use Protein A). Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 1-2 μg/mL for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: CaC1 melanoma cells and melanomas.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA, 0.05 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MLANA

Target: MLANA (Melan A (MLANA))

Andere Bezeichnung: MLANA / Melan-A / MART-1

Hintergrund: Name/Gene ID: MLANA

Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

Gen-ID: 2315

UniProt: Q16655