IRS1 Antikörper (AA 1222-1235) (Biotin)
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- Target Alle IRS1 Antikörper anzeigen
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
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Bindungsspezifität
- AA 1222-1235
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Reaktivität
- Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser IRS1 Antikörper ist konjugiert mit Biotin
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP)
- Spezifität
- Recognizes rat IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
- Homologie
- Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).
- Aufreinigung
- Protein A purified
- Immunogen
- Carboxy terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).
- Isotyp
- IgG
- Top Product
- Discover our top product IRS1 Primärantikörper
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- Applikationshinweise
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Approved: IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects IRS-1 in RIPA lysates from 3T3/A31 mouse fibroblasts. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRS-1 (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: 3T3/A31 cell lysate. Load 20 μg of reduced lysate per lane for minigels. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Kommentare
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Target Species of Antibody: Rat
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.05 % sodium azide, 40 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
- Andere Bezeichnung
- IRS1 / IRS-1 (IRS1 Produkte)
- Synonyme
- HIRS-1 antikoerper, G972R antikoerper, IRS-1 antikoerper, IRS1IRM antikoerper, irs1 antikoerper, irsu antikoerper, IRS1 antikoerper, irs-1 antikoerper, irs1-a antikoerper, insulin receptor substrate 1 antikoerper, insulin receptor substrate 2 antikoerper, insulin receptor substrate 1 S homeolog antikoerper, IRS1 antikoerper, Irs1 antikoerper, irs2 antikoerper, irs1.S antikoerper
- Hintergrund
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Name/Gene ID: IRS1
Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1 - Gen-ID
- 3667
- UniProt
- P35568
- Pathways
- Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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