IRS1 Antikörper (AA 1222-1235) (Biotin)

Produktnummer ABIN226105

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch IRS1 in WB und IP. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für IRS1 Antikörper (AA 1222-1235) (Biotin) (ABIN226105)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRS1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-IRS1 Antikörper (Biotin)

Bindungsspezifität: AA 1222-1235

Spezifität: Recognizes rat IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.

Homologie: Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).

Aufreinigung: Protein A purified

Immunogen: Carboxy terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects IRS-1 in RIPA lysates from 3T3/A31 mouse fibroblasts. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRS-1 (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: 3T3/A31 cell lysate. Load 20 μg of reduced lysate per lane for minigels. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Andere Bezeichnung: IRS1 / IRS-1

Hintergrund: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

Gen-ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process