ACTC1 Antikörper (N-Term)

Produktnummer ABIN224333

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-ACTC1 Antikörper wird verwendet zum Nachweis von ACTC1 in Proben von Human, Kaninchen, Rind (Kuh) und Huhn. Er wurde validiert für WB, IHC, ELISA, IHC (p) und IHC (fro).

Kurzübersicht für ACTC1 Antikörper (N-Term) (ABIN224333)

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reaktivität: Human, Kaninchen, Rind (Kuh), Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ACTC1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue, it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no cross-reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Species cross-reactivity: Human, bovine, rabbit, chicken.

Aufreinigung: Protein G purified

Immunogen: Synthetic N-terminus decapeptide of cardiac isoform of actin.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:500), IHC, IHC-Fr, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 1:10. Frozen and paraffin sections. Note: It is necessary to include 0.5 M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Western Blot: 1:1000 (using the ECL-enhanced procedure). ELISA: 1:500. Note: It is necessary to include 1 M sodium chloride for application in Western blot and ELISA to enhance specificity.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile ddH2O

Konzentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing

Lagerung: -20 °C

Informationen zur Lagerung: Reconstituted or lyophilized: store at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid freeze-thaw cycles.

Referenzen für anti-ACTC1 Antikörper (ABIN224333)

Itier, Ret, Viale, Sweet, Bangari, Caron, Le-Gall, Bénichou, Leonard, Deleuze, Orsini: "Effective clearance of GL-3 in a human iPSC-derived cardiomyocyte model of Fabry disease." in: Journal of inherited metabolic disease, (2014) (PubMed).

Details zu ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Andere Bezeichnung: ACTC1 / Alpha Cardiac Actin

Hintergrund: Name/Gene ID: ACTC1

Synonyms: ACTC1, Actin, alpha cardiac muscle 1, Actin, alpha, cardiac muscle, Actin, alpha, cardiac muscle 1, Alpha-cardiac actin, CMH11, CMD1R, ACTC, LVNC4, ASD5

Gen-ID: 70

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction