ACTC1 Antikörper (N-Term)

Produktnummer ABIN224333

Der Maus Monoklonal Anti-ACTC1-Antikörper wurde für WB, IHC, ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, ACTC1 in Proben von Human, Kaninchen, Huhn und Rind (Kuh) zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für ACTC1 Antikörper (N-Term) (ABIN224333)

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reaktivität: Human, Kaninchen, Huhn, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ACTC1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue, it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no cross-reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Species cross-reactivity: Human, bovine, rabbit, chicken.

Aufreinigung: Protein G purified

Immunogen: Synthetic N-terminus decapeptide of cardiac isoform of actin.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:500), IHC, IHC-Fr, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 1:10. Frozen and paraffin sections. Note: It is necessary to include 0.5 M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Western Blot: 1:1000 (using the ECL-enhanced procedure). ELISA: 1:500. Note: It is necessary to include 1 M sodium chloride for application in Western blot and ELISA to enhance specificity.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile ddH2O

Konzentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing

Lagerung: -20 °C

Informationen zur Lagerung: Reconstituted or lyophilized: store at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid freeze-thaw cycles.

Referenzen für anti-ACTC1 Antikörper (ABIN224333)

Itier, Ret, Viale, Sweet, Bangari, Caron, Le-Gall, Bénichou, Leonard, Deleuze, Orsini: "Effective clearance of GL-3 in a human iPSC-derived cardiomyocyte model of Fabry disease." in: Journal of inherited metabolic disease, (2014) (PubMed).

Details zu ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Andere Bezeichnung: ACTC1 / Alpha Cardiac Actin

Hintergrund: Name/Gene ID: ACTC1

Synonyms: ACTC1, Actin, alpha cardiac muscle 1, Actin, alpha, cardiac muscle, Actin, alpha, cardiac muscle 1, Alpha-cardiac actin, CMH11, CMD1R, ACTC, LVNC4, ASD5

Gen-ID: 70

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction