Cd120b, Tnf-r2 Hp9003 Antikörper

Produktnummer ABIN2192231

Produktdetails

Target: Cd120b, Tnf-r2 Hp9003

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Kreuzreaktivität (Details): Cross reactivity: Human TNF-R1 : Yes,* Human TNF-alpha Minimal crossreactivity problematic in case of TNF-R2 knockout studies

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Antibody reactivity with cell bound or soluble TNF-R2 is not inhibited by high concentrations of human TNFα. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Antigendetails

Target: Cd120b, Tnf-r2 Hp9003

Hintergrund: Type 2 (TNF-R2) of the membrane-bound as well as the soluble receptor. TNF-R2 (~75-80 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-R1 and TNF-R2, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-R1. TNF-R1 is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-R2 is efficiently activated only by the membrane bound form of TNF-alpha. Binding of the inherently trimeric TNF-alpha to TNFR1 and TNFR2 induces receptor trimerization and recruitment of several signaling proteins to the cytoplasmic domains of the receptors. Occupancy of TNFR2 results in direct recruitment of TNF Receptor Associated Factor 2 (TRAF2), which in turn recruits TRAF1. Tumor necrosis factor receptor superfamily member 1B, TNF-RII, p75/p80 Aliases Rabbit IgG