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Muscarinic Acetylcholine Receptor M2 Antikörper (Cholinergic Receptor, Muscarinic 2) Primary Antibody

CHRM2 Reaktivität: Human Func, IA, FACS, IF, WB Wirt: Maus Monoclonal B8E5 unconjugated
Pubmed (2 Referenzen)
Produktnummer ABIN2192224
$659.59
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle Muscarinic Acetylcholine Receptor M2 (CHRM2) Antikörper anzeigen
    Muscarinic Acetylcholine Receptor M2 (CHRM2)
    Reaktivität
    • 93
    • 43
    • 41
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Human
    Wirt
    • 83
    • 11
    • 7
    • 2
    Maus
    Klonalität
    • 90
    • 12
    Monoklonal
    Konjugat
    • 48
    • 7
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser Muscarinic Acetylcholine Receptor M2 Antikörper ist unkonjugiert
    Applikation
    • 64
    • 41
    • 26
    • 26
    • 17
    • 16
    • 16
    • 16
    • 8
    • 6
    • 4
    • 2
    • 2
    • 1
    Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
    Sterilität
    0.2 μm filtered
    Klon
    B8E5
  • Applikationshinweise
    It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.1 % bovine serum albumin.
    Lagerung
    4 °C
  • Nascimento, Sallé, Hoebeke, Argibay, Peineau: "cGMP-mediated inhibition of cardiac L-type Ca(2+) current by a monoclonal antibody against the M(2) ACh receptor." in: American journal of physiology. Cell physiology, Vol. 281, Issue 4, pp. C1251-8, (2001) (PubMed).

    Elies, Fu, Eftekhari, Wallukat, Schulze, Granier, Hjalmarson, Hoebeke: "Immunochemical and functional characterization of an agonist-like monoclonal antibody against the M2 acetylcholine receptor." in: European journal of biochemistry / FEBS, Vol. 251, Issue 3, pp. 659-66, (1998) (PubMed).

  • Target
    Muscarinic Acetylcholine Receptor M2 (CHRM2)
    Andere Bezeichnung
    Muscarinic Acetylcholine Receptor m2 (CHRM2 Produkte)
    Synonyme
    HM2, AChR-M2, Chrm-2, M2, chrm2, Acm2, GPM2, cholinergic receptor muscarinic 2, cholinergic receptor, muscarinic 2, cardiac, cholinergic receptor, muscarinic 2a, cholinergic receptor, muscarinic 2, CHRM2, Chrm2, chrm2a
    Hintergrund
    The monoclonal antibody B8E5 recognizes human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa. This receptor is an integral membrane protein consisting of seven membrane spanning α-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. 2+ Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. M2ACh-R Aliases Peptide VRTVEDGECYIQFFSNAAVTFGTAI(C): Human second extracellular loop residues 168-192 Immunogen Mouse IgG2a
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