For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Haltbarkeit
12 months
Nogee, Wert, Proffit, Hull, Whitsett: "Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 3 Pt 1, pp. 973-81, (2000) (PubMed).
Clark, Wert, Bachurski, Stahlman, Stripp, Weaver, Whitsett: "Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7794-8, (1995) (PubMed).
Gregory, Longmore, Moxley, Whitsett, Reed, Fowler, Hudson, Maunder, Crim, Hyers: "Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome." in: The Journal of clinical investigation, Vol. 88, Issue 6, pp. 1976-81, (1992) (PubMed).
Weaver, Sarin, Sawtell, Hull, Whitsett: "Identification of surfactant proteolipid SP-B in human surfactant and fetal lung." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 65, Issue 2, pp. 982-7, (1988) (PubMed).
Whitsett, Hull, Ohning, Ross, Weaver: "Immunologic identification of a pulmonary surfactant-associated protein of molecular weight = 6000 daltons." in: Pediatric research, Vol. 20, Issue 8, pp. 744-9, (1986) (PubMed).
Target
Surfactant Protein B Cross Reactive with Mouse, Bovine and Pig Sp-B
Hintergrund
The polyclonal antibody recognizes the human surfactant protein B (SP-B). There are four surfactant- specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and