IHC, IP, WB
For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The antibody should be used in TBS and not in PBS.
During complement activation the circulating complement compound C6 is bound by the C3b5b complex to form the first stage of the membrane attack complex. The formed C5b6 complex binds to C7 where after C8 binds, followed by binding of a variable amount of C9 Molecules. The complement factor C6 is an acute-phase reactant for which a CCAAT enhancer binding site essential is.