Cytotoxic Necrotizing Factor 1 Antikörper

Produktnummer ABIN2192151

Produktdetails

Target: Cytotoxic Necrotizing Factor 1

Reaktivität: Chemical

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Dot Blot (DB), Western Blotting (WB), Immunoassay (IA), Inhibition Assay (InhA)

Sterilität: 0.2 μm filtered

Klon: NG8

Anwendungsinformationen

Applikationshinweise: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen

McNichol, Rasmussen, Carvalho, Meysick, OBrien: "Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein." in: Infection and immunity, Vol. 75, Issue 11, pp. 5095-104, (2007) (PubMed).

Meysick, Mills, OBrien: "Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli." in: Infection and immunity, Vol. 69, Issue 4, pp. 2066-74, (2001) (PubMed).

Antigendetails

Target: Cytotoxic Necrotizing Factor 1

Substanzklasse: Chemical

Hintergrund: The monoclonal antibody NG8 is specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin. Monoclonal antibody NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.