For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50..
Nur für Forschungszwecke einsetzbar
PBS, containing 0.1 % bovine serum albumin
Informationen zur Lagerung
Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
Jaillon, Jeannin, Hamon, Frémaux, Doni, Bottazzi, Blanchard, Subra, Chevailler, Mantovani, Delneste: "Endogenous PTX3 translocates at the membrane of late apoptotic human neutrophils and is involved in their engulfment by macrophages." in: Cell death and differentiation, Vol. 16, Issue 3, pp. 465-74, (2009) (PubMed).
Jaillon, Peri, Delneste, Frémaux, Doni, Moalli, Garlanda, Romani, Gascan, Bellocchio, Bozza, Cassatella, Jeannin, Mantovani: "The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps." in: The Journal of experimental medicine, Vol. 204, Issue 4, pp. 793-804, (2007) (PubMed).
The monoclonal antibody MNB1 (previously known as clone 16B5) recognizes human pentraxin 3 (PTX3), belonging to the long pentraxin family. PTX3 is an acute-phase glycoprotein of ~45 kDa with glycosylation accounting for about 10 % of its molecular weight. PTX3 has a complex oligomeric structure with protomers linked to each other by disulfide bonds. PTX3 expression is triggered by inflammatory cytokines, resulting in higher levels of circulating PTX3. Several cell types have been reported to produce PTX3, namely macrophages, endothelial cells, neutrophils and synoviocytes. PTX3 is involved in host defense against pathogen infection, in the regulation of the scavenger activity of macrophages and dendritic cells, and in modulation of complement activity by binding to C1q. Furthermore, PTX3 has been implicated in matrix deposition of cumulus cells. Moreover, PTX3 interacts with other biologically active molecules, causing their functional blockade. This has been demonstrated for fibroblast growth factor-2 (FGF-2), for which PTX3 acts as an inhibitor, leading to inhibition of angiogenesis. PTX3, like other pentraxins C-reactive protein (CRP) and serum amyloid P component (SAP), binds apoptotic cells and debris. PTX3 is useful as an early indicator of myocyte irreversible injury in ischemic cardiomyopathy. PTX3 is not only involved in inflammatory vessel diseases related to atherosclerosis, but also in pre-eclampsia and systemic small vessel ANCA-associated vasulitis, in which neutrophils are key players. The relationship between tissue damage and pentraxin generation is stringent in acute injuries. PTX3 tunes self-non-self discrimination and tissue repair due to the recognition of diverse ligands by PTX3 and through regulation of effector pathways. Aliases Pentraxin-related protein PTX3, Tumor necrosis factor-inducible gene 14 protein, TSG-14 Immunogen Human recombinant PTX3